mouse monoclonal anti mitf antibody  (Thermo Fisher)

Journal: Eye and Vision

Article Title: Ex vivo cultivated retinal pigment epithelial cell transplantation for the treatment of rabbit corneal endothelial dysfunction

doi: 10.1186/s40662-023-00351-4

Figure Lengend Snippet: Antibodies for immunofluorescence staining

Article Snippet: Mouse monoclonal to MITF , Abcam , ab3201 , 1:100.

Techniques: Immunofluorescence

Journal: PLoS ONE

Article Title: A Gene Expression Signature of Invasive Potential in Metastatic Melanoma Cells

doi: 10.1371/journal.pone.0008461

Figure Lengend Snippet: (A) Unsupervised hierarchical clustering by using 96 genes identified by class comparison as differentially expressed between relatively higher or lower MITF -expressing melanoma cell lines classified the cell lines into two main groups (Motif 1, green label; Motif 2, black label). (B) qPCR validation of selected targets in a subset of Motif 1 and 2 NZM cell lines. Unsupervised clustering of qPCR data confirmed the Motif 1 (green) and 2 (black) classifications. (C) Protein expression of MITF targets CDK2, BCL2, and MLANA agreed with MITF transcript levels and array and qPCR cell line stratification. *Motif 1 cell lines. (D) Immunofluorescence showed stronger staining for MITF and MLANA in the Motif 2 NZM06 cells compared to Motif 1 NZM09 cells.

Article Snippet: Coverslips were rinsed with PBS before incubation with 1% BSA in PBS for 30 min. to block non-specific antibody binding, then incubated for 60 min. at room temperature with either mouse monoclonal anti-MLANA antibody, Santa Cruz), or mouse monoclonal anti-MITF antibody (clone C5+D5, Zymed).

Techniques: Expressing, Immunofluorescence, Staining

Journal: BMC Veterinary Research

Article Title: RACK1, a clue to the diagnosis of cutaneous melanomas in horses

doi: 10.1186/1746-6148-8-95

Figure Lengend Snippet: RACK1, CK5 and MITF immunolabelings in horse skin. RACK1 protein labeling (green), MITF (red) and CK5 (magenta) in control horse skin. Cytoplasm of basal keratinocytes is positive for CK5 signal and RACK1 (B). Melanocytes are positive for MITF, but negative for CK5 and RACK1 (A, B). Dotted line indicates epidermis-dermis boundary. e, epidermis; d, dermis. Bar: 10 μm.

Article Snippet: Antibodies were mouse monoclonals anti-MITF (Zymed, dilution 1:50; Invitrogen, Cergy-Pontoise, France) anti-RACK1 (Transduction Laboratories, 1:150; BD Biosciences, Le Pont de Claix, France) and rabbit polyclonals anti- cytokeratin5 (Covance; 1:1000; Eurogentec, Angers, France) and anti-PAX3 (Zymed; 1:200).

Techniques: Labeling

Journal: BMC Veterinary Research

Article Title: RACK1, a clue to the diagnosis of cutaneous melanomas in horses

doi: 10.1186/1746-6148-8-95

Figure Lengend Snippet: RACK1 and MITF immunolabelings in cutaneous melanocytic proliferations from horses. MITF labeling is shown in red and RACK1 in green. ( A ) melanocytoma, ( B ) melanoma, ( C ) achromic melanoma. In melanocytoma, RACK1 cytoplasmic expression is heterogeneous (A1 and A2). Arrowheads point to melanocytic cells that express variable amounts of RACK1. By contrast, in pigmented or achromic melanoma (B1 to C2), all MITF-positive cells display a strong and homogeneous cytoplasmic RACK1 signal (B2, C2). Nuclear counterstaining is shown in blue. Corresponding bright-field photographs are presented (A3 to C3). Magnification is the same in all images, scale bar represents 10 μm.

Article Snippet: Antibodies were mouse monoclonals anti-MITF (Zymed, dilution 1:50; Invitrogen, Cergy-Pontoise, France) anti-RACK1 (Transduction Laboratories, 1:150; BD Biosciences, Le Pont de Claix, France) and rabbit polyclonals anti- cytokeratin5 (Covance; 1:1000; Eurogentec, Angers, France) and anti-PAX3 (Zymed; 1:200).

Techniques: Labeling, Expressing

Journal: BMC Veterinary Research

Article Title: RACK1, a clue to the diagnosis of cutaneous melanomas in horses

doi: 10.1186/1746-6148-8-95

Figure Lengend Snippet: Variability of histological features of horse melanomas and uniformity of RACK1/MITF labeling. ( A - B ): Sections of two different melanomas with hematoxylin-eosin-safran staining, with their respective low magnification in the inserts. Note the variability in pigmentation between tumors ( A, B ) and in different areas of the same tumor (A1, A2). ( C ): Histological staining of a pigmented area from A with ovoid and spindled cells (C1), and low power capture of RACK1 (green) - MITF (red) labeling in the adjacent section (C2), (C3) bright-field corresponding to C2. Note RACK1 signal uniformity in the different areas. Bar: 50 μm.

Article Snippet: Antibodies were mouse monoclonals anti-MITF (Zymed, dilution 1:50; Invitrogen, Cergy-Pontoise, France) anti-RACK1 (Transduction Laboratories, 1:150; BD Biosciences, Le Pont de Claix, France) and rabbit polyclonals anti- cytokeratin5 (Covance; 1:1000; Eurogentec, Angers, France) and anti-PAX3 (Zymed; 1:200).

Techniques: Labeling, Staining

Journal: bioRxiv

Article Title: Glucose oxidation and nutrients availability drive neural crest development

doi: 10.1101/2022.09.05.506657

Figure Lengend Snippet: Immunostaining for Sox-10 (A), qPCR (B), and in situ hybridization (C) analyses of the expression of Sox-10 and Foxd-3 transcripts in delaminating and migrating NCC after 5 h. In A, nuclei are visualized with Dapi. In control and glucose medium, Foxd-3 messages are distributed in premigratory NCC in the dorsal NT (C, arrowheads) as well as in the delaminated cells visible in either sides of the NT (C, arrows) similar to that of Snail-2 . In 2-DG and oligomycin, it is decreased essentially in premigratory cells whereas in pyruvate medium it is reduced only in delaminating cells. (D) Quantification of the proportion of Sox-10 + , HNK-1 + , Tuj-1 + , MITF + , and α-SMA + cells in NCC primary cultures over time under different nutrient conditions. Bar in A = 50 μm. Error bars = S.E.M. Bars in A = 50 μm, and C = 100 μm. Error bars = S.E.M.

Article Snippet: For immunolabeling, the following primary antibodies were used: Rabbit monoclonal antibody (mAb) to Snail-2 (clone C19G7, Cell Signaling, 1/300), mouse mAb to Sox-10 (Clone A2, Santa Cruz, 1/200), mouse mAb to β-catenin (clone 14, BD-Transduction Laboratories, 1/200), mouse mAb to paxillin (Clone 165, BD-Transduction Laboratories, 1/100), mouse mAb to α-SMA conjugated to Cy3 (Clone 1A4, Sigma, 1/300), mouse mAb to βIII-tubulin conjugated to Alexa488 (Clone Tuj-1, R&D Systems, 1/500), mouse mAb to HNK-1 described previously ( , undiluted culture supernatant), mouse mAb to MITF (clone C5, Abcam, 1/200), and mouse mAb to Glut-1 (Ab14683, Abcam, 1/50).

Techniques: Immunostaining, In Situ Hybridization, Expressing, Control